Carbonic Anhydrases from Human Erythrocytes
نویسندگان
چکیده
منابع مشابه
Human Carbonic Anhydrases
Sequence studies of human erythrocyte carbonic anhydrase B show that it contains 256 residues. A series of relatively small peptides soluble in aqueous solutions obtained by chymotryptic and tryptic digestion of the enzyme, large peptides containing only COOH-terminal lysine or arginine as well as those resulting from more limited cleavage of the enzyme, and cyanogen bromide cleavage products s...
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Human carbonic anhydrase B loses enzymatic activity as it undergoes an irreversible conformational change near pH 4 (1). Riddiford (2) further found that seven of the eleven histidyl groups, which are unreactive in the native protein, become titratable during this change. Also, five or six of the eight tyrosyl groups do not ionize freely and reversibly in the native protein (2, 3). Human carbon...
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The more acidic, minor isozymes of human carbonic anhydrase types B and C are present in higher concentrations in the older erythrocyte populations. The levels of all of these isozymes can be determined quantitatively by immunochemical methods following their separation by electiofocusing. A wide variety of human tissues have been assayed for carbonic anhydrases. Significant levels of the C typ...
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THE CONCEPT OF THE TRANSPORT METABOLON A metabolon has been defined as a “temporary, structural-functional, supramolecular complex of sequential metabolic enzymes and cellular structural elements, in which metabolites are passed from one active site to another without complete equilibration with the bulk cellular fluids” (Srere, 1985, 1987). This substrate channeling should decrease transit tim...
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Natural products represent a straightforward source for molecular structures bearing a vast array of chemical features and potentially useful for biomedical purposes. Recent examples of this type include the discovery of the coumarins and the polyamine natural products as atypical chemotypes for the inhibition of the metalloenzymes carbonic anhydrases (CAs; EC 4.2.2.1). CA enzymes are establish...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1964
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)91392-x